Characterization of Photoswitchable Sodium Channel Inhibitors by Planar Patch Clamp

Automated planar patch-clamp.

Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as controlling many metabolic functions. Furthermore, i...

Multichannel incubation type planar patch clamp biosensor using plastic (PMMA) substrates and characterization by laser-gated ion-channel protein

Recently we have developed 4 channel incubation type planar patch clamp biosensor with polymethylmethacrylate (PMMA) substrate by using both-side hot embossing, focused ion beam micro-fabrication and surface modification by extra cellular matrix. We have evaluated the performance by using the light-gated ion-channel protein, channelrhodopsin wide receiver (ChRWR) expressed on HEK293 cell. Obser...

One-channel cell-attached patch-clamp recording.

Ion channel proteins are universal devices for fast communication across biological membranes. The temporal signature of the ionic flux they generate depends on properties intrinsic to each channel protein as well as the mechanism by which it is generated and controlled and represents an important area of current research. Information about the operational dynamics of ion channel proteins can b...

expression of potassoim channel in stem cell derived-cardiomyocytes evaluated by patch-clamp technique

Identifying modulators of hERG channel activity using the PatchXpress planar patch clamp.

The authors used the PatchXpress 7000A system to measure compound activity at the hERG channel using procedures that mimicked the "gold-standard" conventional whole-cell patch clamp. A set of 70 compounds, including hERG antagonists with potencies spanning 3 orders of magnitude, were tested on hERG302-HEK cells using protocols aimed at either identifying compound activity at a single concentrat...